Additive Manufacturing Technologies (AMT)
> Zum Inhalt

MatureTissue

We are delighted to announce a new doctoral college between the TU Wien and the Technikum Wien: the MatureTissue

DC Mature Tissue | MatureTissue - Joint DC by UASTW and TUW dc-mature-tissue.at

 

MatureTissue – Driving maturation of artificial tissues using bioreactor and microfluidic technologies

The particular scientific focus of MatureTissue is to bundle existing technologies available at UASTW and TUW in the fields of bioreactors/biomaterials, microfluidics, 3D printing and microscaffolds, omics technologies and biomechanics towards establishing models of mature musculoskeletal tissues, specifically of tendon, muscle, cartilage and bone.

 

 

Within this programme, our group offers 1 full-time PhD position in the field of Spheroids for Biofabrication.

 

PhD Topic 5: Microscaffold-reinforced Stem Cell Based Spheroids as Building Blocks for Modular Tissue Engineering

 

Hypothesis/Aim: Due to high cell density and biomimetic conditions, spheroids are increasingly used as building blocks in tissue engineering. Nevertheless, merging multiple spheroids together results in final macrotissue assemblies, with poor control over their viability, their morphological shape and volume and their mechanical properties. Reinforcing them with biodegradable microscaffolds, produced by means of high-resolution 3D printing (i.e multi-photon polymerization [1,2]), provides unprecedented possibilities to precisely tune and dictate such properties. Multi-photon polymerization will be used to print microscaffolds of various designs in terms of diameter, porosity and shape. Stem cells suspension, such as human adipose derived stem cells, will be cultivated under low binding condition with and without microscaffolds until spheroids are formed. At the end of the differentiation period, cell viability (Live and Dead), ECM production (immunohistology, biochemical assays) and mechanical properties of the spheroids (AFM) will be tested. Then, the capacity of spheroids to self-assemble into larger macrotissues along with their stability and maturation over-time will be assessed. This self-assembly and fusion of single spheroids to larger tissue-like structures shall be enhanced by compression. Therefore the PhD candidate will use different hydrostatic pressure bioreactor systems available at UASTW (one published [4] and one unpublished custom-made system, and a commercial Flexcell® Compression system [5]) and investigate the impacts of HP on the functionality of those stem cell-based spheroids. We aim that with the addition of controlled mechanical stimulation via bioreactor systems we can achieve major progresses and stay pioneers in this “Third Tissue Strategy in Tissue Engineering”.

 

References:  [1] Angew Chem Int Ed Engl. 2018 [2] Adv Healthc Mater. 2020 Aug;9(15):e1900752.

[3] Biofabrication. 2020 Mar 31;12(2):025033 [4] Sci Rep. 2018 Nov 19;8(1):17010.

[5] https://www.flexcellint.com/category/compression

 

 

Any interest, then apply here for the project 5!


Application | DC Mature Tissue (dc-mature-tissue.at)